Chenguang Fan

Chenguang Fan

Assistant Professor, Biochemistry

J. William Fulbright College of Arts & Sciences

(CHBC)-Chemistry & Biochemistry

Phone: 479-575-4653

Fax: 479-575-4049

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The research interest in our group includes protein biochemistry, microbiology, and synthetic biology. The major approach is to use genetic code expansion technique to incorporate noncanonical amino acids into proteins for different studies:

1) Protein biochemistry: developing noncanonical amino acid incorporation systems for labeling proteins, studying post-translational modifications, and mapping protein-protein interaction networks.

2) Microbiology: applying the genetic code expansion technique in Salmonella, one of the most widely distributed pathogens. Specific topics include studying effects of post-translational modifications in Salmonella toxicity, exploring host targets of Salmonella toxins, and designing small molecules to block Salmonella metabolic organelle functions.

3) Synthetic biology: utilizing naturally designed protein complexes in bacteria as nano-bioreactors for biofuel and chemical compound production.

Postdoctoral associate, Yale University

PhD, Iowa State University

Selected Publications

1.  Fan C*, Ip K, Söll D*. Expanding the genetic code of Escherichia coli with phosphotyrosine. FEBS letters. 2016 [Epub ahead of print]

2.  Xiong H, Reynolds N*, Fan C* et al. Dual genetic encoding of acetyl-lysine and non-deacetylatable thioacetyl-lysine mediated by flexizyme. Angew. Chem. Int. Ed. Engl. 2016;55(12):4083-4086.

3.  Fan C*, Xiong H, Reynolds N, and Söll D*. Rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids. Nucleic Acids Res. 2015;43(22):e156.

4.  Amiram M, Haimovich A, Fan C et al. Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. Nat. Biotechnol. 2015;33(12):1272-1279.

5.  Fan C*, Ho J*, Chirathivat N, Söll D, and Wang Y-S. Exploring the substrate range of wild-type aminoacyl-tRNA synthetases. ChemBioChem. 2014;15(12):1805-1809.

6.  Fan C, Cheng S, Sinha S and Bobik TA. Interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments. Proc. Natl. Acad. Sci. USA. 2012;09(37):14995-15000.

7.  Cheng S, Fan C, Sinha Sand Bobik TA. The PduQ enzyme is an alcohol dehydrogenase used to recycle NAD+ internally within the Pdu microcompartment of Salmonella enterica. PLoS One. 2012;7(10):e47144.

8.  Fan C and Bobik TA. The N-terminal region of the medium subunit (PduD) packages adenosylcobalamin-dependent diol dehydratase (PduCDE) into the Pdu micro-compartment. J. Bacteriol. 2011;193(20):5623-5628.

9.  Fan C, Cheng S, Liu Y et al. Short N-terminal sequences package proteins into bacterial microcompartments. Proc. Natl. Acad. Sci. USA. 2010;107 (16):7509-7514.

10.  Fan C, Fromm HJ, and Bobik TA. Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella enterica. J. Biol. Chem. 2009;284(30): 20240-20248.

11.  Fan C and Bobik TA. The PduX enzyme of Salmonella enterica is an L threonine kinase used for coenzyme B12 synthesis. J. Biol. Chem. 2008;283(17):11322-11329.

12.  Fan C and Bobik TA. Functional characterization and mutation analysis of human ATP:Cob(I)alamin adenosyltransferase. Biochemistry. 2008;47(9):2806-2813.